CHANGES IN MICROTUBULES OF CILIA AND FLAGELLA FOLLOWING NEGATIVE STAINING WITH PHOSPHOTUNGSTIC ACID

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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The Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid Lloyd

After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...

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ژورنال

عنوان ژورنال: The Biological Bulletin

سال: 1970

ISSN: 0006-3185,1939-8697

DOI: 10.2307/1540082